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30 years of cell culture excellence

Author: Dan Haus

Choosing the Right hPSC Medium – Points to Consider

Today, when it comes to choosing which human pluripotent stem cell (hPSC) medium to use for your stem cell cultures, scientists and clinicians have many choices. You may be asking yourself “How do I know which media I should be using?” or “How do I choose the right media for me?” In this blog post, I will be discussing a few considerations to keep in mind when choosing a medium that best fits your needs: your application, your chosen substrate(s), the overall compatibility, and ease-of-use. Application When reviewing the various options available in hPSC culture media, researchers often wonder if...

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Observations from the Field: Efficient hiPS cell culturing without ROCK inhibitors

Introduction Stem cell research undoubtedly has the potential to benefit many people with unmet medical needs. However, to fully reach this potential, improvements need to be made in several areas. One such area is cloning efficiency and cell survival after thawing and passaging human pluripotent stem cells, or more generally, improving the ability to generate and subsequently propagate and passage hES and hiPS cells. To address this, RIKEN scientists screened various chemical compounds added to cell culture media to identify pro-growth and survival factors. The result was the identification of the small molecule Y-27632, a selective inhibitor of Rho-associated coiled-coil kinase...

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