The Evolution of Human Pluripotent Stem Cell Culture Systems (Originally posted on RegMedNet February 2017) Human pluripotent stem (hPS) cells can proliferate for years or more as self-replicating, unspecialized cells, yielding an exponentially expanding number of stem cells in culture – each with the ability to differentiate into any cell or tissue in the body. These attributes make hPS cells a unique tool in the field of regenerative medicine, offering potential to heal damaged tissue and treat cell-based diseases, such as heart disease, diabetes, and spinal cord injuries. Pluripotent stem cells are also commonly used in drug discovery, toxicity...Read More
Author: Lia Kent
In a recent blog post, we summarized two key papers by Rodin et al. (1, 2) on clonal derivation and single-cell passaging of human pluripotent stem (hPS) cells in a completely defined, xeno-free environment using Laminin-521 matrix and NutriStem hPSC Medium. These publications give a wealth of information for hPS cell culture in this system and provide detailed protocols for passaging as both single cells and cell aggregates. Here we highlight some important and practical tips from the papers for culturing hPS cells in NutriStem hPSC Medium with Laminin-521 as the basement matrix: Thawing a frozen vial of Laminin-521...Read More
A guest blog by Dr. Miranda Yang, Stem Cell Researcher Introduction The use of purified laminin protein as a basement matrix for cell culture isn’t exactly new, but recent advances are generating quite a buzz in the stem cell field. This summary is presented by Dr. Miranda Yang, reviewing two key publications by Sergey Rodin and colleagues at the Karolinska Institute in Stockholm, Sweden. These articles published in Nature highlight the long-term culture and clonal propagation of human pluripotent stem (hPS) cells in a completely defined system of NutriStem® hPSC Medium and Laminin-521 matrix. Papers Monolayer culturing and cloning...Read More
A guest blog by Lia Kent, Scientific Training, Biological Industries (BI) recently published on The Cell Culture Dish blog Introduction For over 30 years, Biological Industries has been developing specialized cell culture media for many cell types, including primary cells and stem cells. Over time we have seen a significant evolution of stem cell media as well as our ability to understand, evaluate, and optimize cell culture conditions. Cell culture is one of the most common and complex techniques used in the life sciences, and the media itself is a critical element for maintaining healthy, proliferating stem cells in...Read More
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