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30 years of cell culture excellence

Author: Dan Haus

Observations from the Field: Evaluation of Human Platelet Lysate and Serum-Free Media as Alternatives to FBS for Human MSC Culture

Comparison study showing the growth and expansion of adipose-derived hMSCs using a variety of media, supplements, and attachment substrates As more human mesenchymal stem/stromal cell (hMSC)-based therapies move closer to the clinic, the culture conditions used to propagate these cells in vitro are increasingly coming into the spotlight. We see this reflected every day by the growing number of labs now transitioning their standard culture systems to an environment that better supports the translational goals of their research. Specifically, researchers are seeking cell culture reagents that are produced under cGMP guidelines, consistent from lot-to-lot, and able to meet the...

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Choosing the Right hPSC Medium – Points to Consider

Today, when it comes to choosing which human pluripotent stem cell (hPSC) medium to use for your stem cell cultures, scientists and clinicians have many choices. You may be asking yourself “How do I know which media I should be using?” or “How do I choose the right media for me?” In this blog post, I will be discussing a few considerations to keep in mind when choosing a medium that best fits your needs: your application, your chosen substrate(s), the overall compatibility, and ease-of-use. Application When reviewing the various options available in hPSC culture media, researchers often wonder if...

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Observations from the Field: Efficient hiPS cell culturing without ROCK inhibitors

Introduction Stem cell research undoubtedly has the potential to benefit many people with unmet medical needs. However, to fully reach this potential, improvements need to be made in several areas. One such area is cloning efficiency and cell survival after thawing and passaging human pluripotent stem cells, or more generally, improving the ability to generate and subsequently propagate and passage hES and hiPS cells. To address this, RIKEN scientists screened various chemical compounds added to cell culture media to identify pro-growth and survival factors. The result was the identification of the small molecule Y-27632, a selective inhibitor of Rho-associated coiled-coil kinase...

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